返回 2008年第31卷第1期 目录

肺癌细胞株c-erbB-2基因与放疗抵抗的相关关系*

曹新梅，任德莲，石华山，杨庆龙1，陈 枫2，刘代玉3

（泸州医学院:免疫教研室;1附属医院:肿瘤科;2传染科;3血液内科，四川泸州 646000）

摘 要 目的：探讨人类表皮生长因子受体-2（c-erbB-2）基因与非小细胞肺癌细胞株放疗抵抗的关系。方法：采用RT-PCR测定肺腺癌（CALU-3和A549）和鳞癌NCL-H520细胞株的c-erbB-2 mRNA水平；采用流式细胞术检测CALU-3、A549和NCL-H520细胞株的c-erbB-2蛋白水平；绘制CALU-3、A549和NCL-H520细胞株的存活曲线。结果：肺腺癌CALU-3细胞株的c-erbB-2 mRNA水平均高于腺癌A549和鳞癌NCL-H520细胞株，差异有统计学意义(均P<0.05)，而A549和NCL-H520细胞株之间差异无统计学意义(P>0.05)；CALU-3细胞株c-erbB-2蛋白的阳性率均高于A549和NCL-H520细胞株，差异有统计学意义(均P<0.05)，而A549和NCL-H520细胞株之间差异无统计学意义(P>0.05)。存活曲线显示, CALU-3、A549和NCL-H520细胞株D0值依次为1.18 Gy±0.05 Gy、1.67 Gy±0.13Gy和1.50 Gy±0.26Gy，细胞株间差异无统计学意义(F=0.287,P> 0.05),Dq值分别为3.98 Gy±0.03Gy、2.43 Gy±0.47Gy和1.32 Gy±0.08 Gy (F=61.670,P<0.05)，其中CALU-3和A549、A549和NCL- H520、CALU-3和NCL-H520细胞株之间差异有统计学意义（P分别为<0.05、<0.05、<0.01）。结论：肺腺癌CALU-3细胞株的c-erbB-2基因在mRNA和蛋白水平均高于腺癌A549和鳞癌NCL-H520细胞株；三种细胞株中，CALU-3对γ射线最不敏感， NCL-H520最敏感；推测c-erbB-2基因高表达可能与肺腺癌CALU-3细胞株放疗抵抗有关。

关键词 肺癌；c-erbB-2；放疗

*基金项目：四川省教育厅自然科学科研项目（项目编号：2005A065）

 

RELATIONSHIP BETWEEN C-ERBB-2 GENE OF LUNG CANCER CELL LINES AND THE RESISTANCE TO RADIATION

Cao Xinmei, et al

Department of Immunology, Luzhou Medical College

Abstract Objective:To investigate the relationship between epidermal growth factor receptor-2 (c-erbB-2) of non-small cell lung cancer cell lines and its resistance to radiation.Methods: The c-erbB-2 mRNA level of the human lung adenocarcinoma cell lines CALU-3 and A549 and squamous cell carcinoma NCL-H520 cell line was assayed with RT-PCR；the c-erbB-2 protein expression of the three cell lines was measured with flow cytometry；and the parameters of survival curves of the cell lines was measured.Results: The c-erbB-2 mRNA relative expression in CALU-3 cell line was 0.77±0.07,significantly higher than those in the cell lines A549(0.65±0.05, P<0.05) and NCL-H520(0.62±0.05,P<0.05). There was unconspicuous statistical significance in the difference between A549 and NCL-H520 cell line(P>0.05). The c-erbB-2 protein positive expression rate was 33.78%±13.45% in the cell line CALU-3,significantly higher than that in the cell lines A549(15.60%±6.37%,P<0.05) and NCL-H520(4.22%±0.10%, P<0.05).There was no significant difference between A549 and NCL-H520 cell line (P>0.05). The D0 of the three cell lines was similar(F=0.287,P>0.05),while the Dq of the three cell lines was significantly different(F=61.670,P<0.05). The Dq of CALU-3 cell line was 3.98 Gy±0.03 Gy, higher than that of A549 cell line (2.43 Gy±0.47 Gy,P<0.05) and NCL-H520 cell line (1.32 Gy±0.08 Gy,P<0.01);in addition, the Dq of the other two cell lines was also significantly different(P<0.05).Conclusions:The level of c-erbB-2 mRNA and protein in CALU-3 cell line was higher than that in A549 and NCL-H520 cell line,respectively. Among the three cell lines, CALU-3 cell line was more resistant to the γ-rays than the others, and NCL-H520 cell line was the most sensitive. The high expression of c-erbB-2 gene is probably related to the radioresistance of CALU-3 cell line.

Key words Lung neoplasms;C-erbB-2 gene;Radiation