返回
2007年第30卷第2期 目录
丹参酮ⅡA磺酸钠对单侧输尿管梗阻病人肾间质成纤维细胞Cyclin
D1蛋白
表达的影响及意义*
孙兴旺，曹 灵1 ，于国华，张 弦，郭庆喜，郭 勇2
（泸州医学院：病理学教研室；1附属医院肾内科；2组织胚胎学教研室，四川泸州 646000）
摘 要 目的：研究丹参酮ⅡA磺酸钠（sodium tanshinoneⅡ-A sulfonate，DS-201）对单侧输尿管梗阻(UUO)病人肾间质纤维化来源的成纤维细胞(hRIFs)体外增殖及细胞周期素D1（cyclin
D1）蛋白表达的影响，探讨该药治疗肾间质纤维化的作用机制。方法：体外培养鉴定hRIFs；用四甲基偶氮唑（MTT）法检测对照组与不同DS-201浓度组hRIFs的增殖活性；用免疫细胞化学SABC法和图像分析技术检测对照组与不同DS-201浓度组hRIFs
cyclin D1基因的表达。结果：随药物浓度的升高和作用时间的延长，抑制率逐渐升高，抑制作用逐渐增强，呈剂量依赖性和时间依赖性；用药组阳性细胞的光密度值在第3、5、7、9天显著低于对照组（P＜0.05，P＜0.01﹚，第1天各组之间无统计学差异（P＞0.05）。结论：①
DS-201对UUO病人hRIFs体外增殖有显著抑制作用，可能是其治疗肾间质纤维化的机制之一；② DS-201抑制UUO病人hRIFs体外增殖可能是通过抑制细胞中cyclin
D1基因表达，延长细胞周期实现的。
关键词 丹参酮ⅡA磺酸钠；UUO；纤维化；肾间质；成纤维细胞；细胞周期素D1
*基金项目：四川省中医药管理局科研基金（200022）
EFFECT OF SODIUM TANSHINONE II-A SULFONATE ON HUMAN RENAL INTERSTITIAL
FIBROCYTES OF UUO AND THE EXPRESSION OF CYCLIND1
Sun Xingwang, et al
Department of Pathology, Luzhou Medical College
Abstract Objective: To discuss the effect of
sodium tanshinone II-A sulfonate (DS-201)on human renal interstitial
fibrocytes(hRIFS) of UUO and the expression of cyclin D1 and
approach the possible mechanism. Methods:HRIFs were assessed
through culture in vitro;the propagate activity of HRIFs of
control group and different concentration group of DS-201 was
detected by MIT method.The expression of cyclin D1 were analyzed
with immune cytochemistry SABC and the image-analytical technique.Results:With
the increase of drug concentration and action time,the inhibition
ratio and strength increased,and it was dose-dependent and time-dependent.
The average optical density of positive cell of drug-using group
was much less than control group on the third ,fifth, seventh,
and ninth day（P＜0.05，P＜0.01).There were no significant differences
between groups on 1st day （P＞0.05）. Conclusions: ①DS-201 can
significantly suppress in vitro generation of hRIFs，this may
be one of its mechanisms to treat renal interstitial fibrosis.
②DS-201 suppress in vitro generation of hRIFs is possibly through
inhibiting the expression of cyclin D1 and extending the cell
cycle.
Key words Sodium tanshinone II-A sulfonate;
UUO;Fibrosis;Renal interstitium;Fibrocyte;Cyclin D1
